Colorectal cancer case study. Jurnalul de Chirurgie

Purpose: The phytochemical profile and anticancer potential of three Ajuga sp.

Materials and Methods: The phytochemicals were extracted from the aerial parts of Ajuga sp. The phytochemical profile was also evaluated by principal component analysis in connection with antitumor efficacy of extracts.

Western Blot with regard to inflammatory protein NF-κB nuclear factor kappa-light-chain-enhancer of activated B cells p65 subunit expression in cell lysates was performed. Enzymatic and non-enzymatic antioxidant capability was assessed by measuring catalase activity and by evaluating the total antioxidant capacity TAC of treated cells.

Results: Ajuga laxmannii ethanol extract showed the highest total phenolic and flavonoid content, while A.

Implicaţiile microbiomului în iniţierea şi promovarea carcinogenezei

The overall cytostatic effect of the investigated plant extracts was exerted through strong colorectal cancer case study actions on NF-κB, the key molecule involved in the inflammatory response and via oxidative stress modulatory effects in both murine colon carcinoma and melanoma cell lines. Conclusion: Ajuga laxmannii showed the most significant antitumor activity and represents an important source of bioactive compounds, possibly an additional form of treatment alongside conventional anticancer drugs.

Case 1: BRAF-Mutated Metastatic Colorectal Cancer

Introduction Medicinal plants have always been an important source for various pharmaceuticals since ancient times. Nowadays the scientific interest for new drugs production from bioactive compounds isolated from natural products is still growing. Herbal medicines were analize oxiuri used only based on empirical observations since antiquity, without knowing the phytochemicals from the extracts or colorectal cancer case study of their pharmacological effects Atanasov et al.

Although many herbal remedies have a well-known composition and certain biological effects, some of them are still used only based on traditional medicine, and lacking the validation of their safety and efficacy.

COLONOSCOPIC SCREENING OF ASYMPTOMATIC FIRST-DEGREE RELATIVES OF COLORECTAL CANCER PATIENTS

The research on unexplored medicinal plants traditionally used in folk medicine could determine the development of novel herbal formulations with significant biological activities. Due to their important pharmacological effects, the natural compounds are effectively used to obtain new phytomedicines. Ajuga species Lamiaceaewhich are widely distributed in many parts of the world Atay et al. Six Ajuga species are mentioned in the Romanian spontaneous flora, with Ajuga genevensis L.

Our previous research showed the antioxidant, antimicrobial, and anti-inflammatory effects of aerial parts extracts Toiu et colorectal cancer case study. The monoterpene glycosides content and colorectal cancer case study essential oil composition have been recently studied on species from Italy, together with the evaluation of antioxidant activity and cytotoxicity by MTT assay Venditti et al.

Implicaţiile microbiomului în iniţierea şi promovarea carcinogenezei

The anticancer activity of natural compounds is attributed to their synergistically acting complex mixture of phytochemicals with chemopreventive and chemotherapeutic potential, which can prove to be far more effective than isolated bioactive molecules de Kok et al.

Accordingly, the unexplored plants used in folk medicine require extensive studies for reliable evidence-based phytotherapy.

Although complementary and alternative ethnopharmacological approaches are mainly focused on counteracting the side effects and collateral symptoms of conventional cancer therapies, in this paper we investigated a potential disjunction change in traditional plant use Leonti and Casu,by assessing the anticancer activity of these indigenous herbs.

Therefore, this study was aimed to perform a comparative phytochemical analysis of A.

F10 murine melanoma and C26 colon carcinoma cells. Both cell lines are characterized by increased metastatic potential and are prone to therapeutic alterations of their redox status Rauca et al.

In addition, melanoma and colon carcinoma are two of the deadliest cancers in modern society, possibly interlinked by epigenetic mechanisms, as recent reports concluded that colorectal cancer is one of the most common discordant cancers post-melanoma Frank et al.

Desfășurăm experimente esentiale

As previously reported, the highly metastatic B The potential synergistic interaction between bioactive constituents suggests that the whole plant extract may contribute to better therapeutic outcomes compared to the administration of single isolated compounds at an equivalent dose Rasoanaivo et al. Materials and Methods Chemicals and Reagents High purity chemicals: sodium carbonate, sodium acetate trihydrate, and anhydrous aluminum chloride were acquired from Sigma-Aldrich Germany.

Folin-Ciocâlteu colorectal cancer case study was purchased from Merck Germany. HPLC grade solvents methanol, acetonitrile, ammonium acetate, and silver nitrate were purchased from Sigma-Aldrich. Preparation of Standard Solutions Standard stock solutions of the flavonoids and iridoids were prepared by dissolving 1 mg of each compound in 1 mL methanol and stored at 4°C, protected from daylight.

We Just Won a Skirmish in the War on Cancer

They were appropriately diluted with double distilled water before being used as working solutions. Plant Samples and Extraction Procedures The aerial parts of Ajuga species collected in flowering stage in June were obtained and authenticated by one of us A.

The dried plant samples were ground to a fine powder before extraction. The aerial parts extracts of A. The liquid chromatograph was equipped with binary gradient pump, degasser, column thermostat and autosampler.

• In press Papers accepted for publication 1.
• Romania Cancer Oranisations and Resources | CancerIndex

The chromatographic separation was performed on a reversed-phase Zorbax SB-C18 mm × 3.